Yes, the gain from your Canadian property is taxable on your US tax return.
The US taxes both US and Foreign source income. You will need to include the gain on the sale of your Canadian property as income on your return and you can claim a Foreign Tax Credit on the Canadian taxes paid on the sale.
See the following regarding claiming the Foreign Tax Credit in TurboTax:
Filing IRS Form 1116 to Claim the Foreign Tax Credit
See the following for additional information on using the US Canada Tax Treaty: