Yes, it could make a difference if you did not also enter the overtime manually after the W-2 input and you qualified for the deduction.
The directions for reporting overtime if it's not on your W-2, is to enter the W-2 and continue through the screens until you reach Let's check for other situations. Check the box for Overtime and select Continue. Enter the total of your qualified overtime. TurboTax will calculate your deduction.
If you missed labeling it as overtime on Box 14 and did not report it on the Let's check for other situations screen, you can amend your return.
Here is how to amend a return to update for the overtime, from How do I amend my federal tax return for a prior year?:
"In TurboTax Online:
1. On the Tax Home screen, scroll down to Your tax returns & documents and select 2025.
2. Select the Amend (change) return dropdown, then Amend using TurboTax Online. If you're told to amend your return via TurboTax Desktop, follow the steps for TurboTax Desktop instead.
3. Continue through the screens, make the changes you need to make, and carefully answer the remaining questions to finish amending your return."
