Can I deduct my annual credit card fees which are used almost exclusively for my work (non-reimbursed). I.e AmEx fee which is used due to rental car insurance coverage.

[kerriP]

For a work credit card, fees that are incurred are deductible.  The question would be "did I get this credit card because of my business and do I use it for my business with little personal (or none for the IRS) use."  Per the IRS: To be deductible, a business expense must be both ordinary and necessary. An ordinary expense is one that is common and accepted in your trade or business. A necessary expense is one that is helpful and appropriate for your trade or business. This link has  some more Information.

Business expenses

[wyblea]

Does this same thing apply if I am an employee? In my case a professor who travels weekly. The credit cards are both "personal," meaning not a business card per se, but the entire reason that I have them is due to the business perks; meaning, that the AmEx is due to the rental car insurance coverage.

[kerriP]

As an employee as long as necessary and are not  reimbursed These expenses are miscellaneous itemized deductions on schedule A.  These expenses must total more than 2% of your AGI  to start counting as deductible.