Yes, you can use the preschool tuition to claim the Child and Dependent Care Credit. See more below.
https://ttlc.intuit.com/replies/4396093
To Enter the Credit in TurboTax Online:
1. Go to "Federal Taxes" at the top of the screen
2. Select "Deductions & Credits"
3. Scroll down to "You and Your Family" and select
"Show More"
4. Select "Start" next to "Child and
Dependent Care Credit"
5. Select "Yes" to "Did you pay for child or
dependent care in 2016?"
6. Select "Continue" on the Add Dependents page and
proceed to enter your info for the credit