Most likely not. When health insurance premiums are deducted from a paycheck by an employer, the employer usually deducts the premiums out of your wife's taxable wages on her W-2 (in Box 1). If this is the case, you cannot take a deduction on the tax return for the insurance premiums because this would create a double tax benefit.
However, if the premiums come out of her pay check after tax (the employer doesn't deduct the premiums from her wages) they would be deductible as an itemized medical expense deduction (not the self-employed health insurance deduction).
See the following from IRS Publication 502:
Employer-Sponsored Health Insurance Plan
Don't include in your medical and dental expenses any insurance premiums paid by an employer-sponsored health insurance plan unless the premiums are included on your Form W-2, Wage and Tax Statement. Also, don't include any other medical and dental expenses paid by the plan unless the amount paid is included on your Form W-2.
Example.
You are a federal employee participating in the premium conversion plan of the Federal Employee Health Benefits (FEHB) program. Your share of the FEHB premium is paid by making a pre-tax reduction in your salary. Because you are an employee whose insurance premiums are paid with money that is never included in your gross income, you can't deduct the premiums paid with that money.
