No, you cannot use TurboTax Online to file for 2016. TurboTax Online is only for the current tax year, which is now 2018. To file for 2016 you have to use the CD or download TurboTax software for 2016. You can purchase a download at the following link.
https://turbotax.intuit.com/personal-taxes/past-years-products/2016/
You might get a better price from a reputable online seller that still has TurboTax for 2016 in stock.
Tax returns for past years cannot be e-filed. You will have to print your 2016 tax return, sign it, and file it by mail.