If you are referring to paying TurboTax software fees with two credit cards, then - no - the processing system will only allow for one credit card.
If you are referring to your taxes due, then you won't be able to use two different cards to make a tax payment through Turbotax. However, you can use the IRS site to make two separate card payments.
Inside TurboTax, you should select “pay by check” during the filing process. It will produce a payment voucher for you, which you can then ignore and make the credit card payments online later.
You can use this link below to make payments by credit card. https://www.irs.gov/payments