No, you can use TurboTax Online, but the cost segregation analysis isn't a tax form that you can fill out in any program.
It's a method of accelerating depreciation deductions by breaking down depreciable property into its smallest components and depreciating them individually.
For example, if you buy a house to rent out, normally you would list the entire property as a single asset depreciable over 27.5 years.
In the cost segregation method, you would list the different components as separate assets and assign a portion of the total cost to each component. See the spread sheet below for an example.
If you want to do this, you need to create a spreadsheet similar to the one below and make sure your totals add up to the amount you paid for the entire property. Then you enter each asset separately in TurboTax.