It depends. Is your charitable donation through work reported on your W-2? Check for an amount in Box 14. If you see it, make sure the Box 14 category is Charitable cash contribution.
TurboTax will automatically move the amount from your W-2 Box 14 to Form 1040. However, the program will ask you to confirm the amount of your payroll deduction.
If your contribution did not appear on your W-2, enter the amount in Deductions & Credits.
Learn more at Most taxpayers can deduct up to $300 in charitable contributions without itemizing deductions.