Can I deduct a 325.00 class required by state (WV) to keep my teacher certification?

Since these expenses were directly related to employment, they may be deductible as job-related expenses. 

Unreimbursed job-related expenses are deductible on Schedule A (Itemized Deductions) and are subject to the 2% floor for miscellaneous itemized deductions.  To input your job-related expenses (TurboTax Deluxe edition or higher is required to file itemized deductions):

https://ttlc.intuit.com/replies/4800418