No. The software will not know if you already used a W-2. TurboTax is software for a do-it-yourself tax return. Look carefully at your W-2. It has the year on it. You should not be entering a W-2 from any other year in to your 2018 tax return, if that is what you are working on. And employers have not even issued 2018 W-2's yet--they are issued in January. Please explain what you are trying to work on.