If your employer allows it, yes.
However, if you employer allows it, your spouse is NOT eligible for contributions to her HSA, even if you choose NOT to use the FSA on her or the dependents. If it is "allowed", she is covered by "other insurance", which disqualifies contributions to the HSA. The exception for that is if the FSA is a "limited" FSA that can only be used for things like dental and vision expenses.
