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Which penalty?  There's an underpayment penalty on 1040 line 38.    That is already included in the tax due on line 37 or reduced a refund on line 35a.   

 

Or did you get a 1099R?  There is a 10% Early Withdrawal Penalty if you are under 59 1/2.  The 10% Early Withdrawal Penalty is automatically included in your tax return and tax due (or reduces your refund). It will break out and show the 10% penalty separately on your return (schedule 2 line 6 which goes to 1040 line 23). Then you get credit for all the withholding taken out on 1040 line 25b.

 

Or how did it prompt you to pay a penalty?  Any penalties in Turbo Tax are included in your tax return.  You don't pay them separately.  Or did the IRS send you a letter for it?