Get your taxes done using TurboTax

Did you mean you are using the 2021 CD Desktop program?  No it's not a deduction.  Enter it like you got a 1099R distribution under Income.  It is not a ROTH IRA contribution.  It is a rollover or conversion.  If your 401K was pretax it will all be taxable at your regular income bracket and may push you into a higher bracket.  

Enter a 1099R under

Federal Taxes Tab or Personal (Home & Business)

Wages & Income at the top

 

Then scroll way down to Retirement Plans and Social Security,

Then IRA, 401(k), Pension Plans (1099R) - click Start or Revisit