When you withdraw funds from a Traditional IRA/401K, you will pay taxes on it. If you are 59 1/2 years or older you would not have to pay penalty on the withdrawal. As you currently own the home you are trying to sell, you will not qualify for a new home exclusion from penalty on the first $10,000.00. This exclusion from penalty is only available once. Though taxes will be owed on the full amount withdrawn from Traditional IRA/401K.
Also remember, you may owe taxes on the sale of your existing home. The exclusion amount for capital gains tax is on first $250,000 if single filer or married filing separately or $500,000 if married filing jointly.
Here is a link to help you understand if the 10% additional tax/penalty for early withdrawal and tax on withdrawal from a retirement account will apply to you.
IRS: Exception to tax on early Withdrawal
IRS: Withdrawals from a retirement account
