Yes, if TurboTax asks for your year-end balance in Roth IRAs you must enter it. TurboTax will ask for your year-end balance in Roth IRAs if your entries show that you have an excess contribution in your Roth IRAs. The excess-contribution penalty is calculated on the lesser of the amount of the excess and the year-end balance.
Examine Schedule 4 line 59 for any penalty from Form 5329.
I'm not aware of any other situation where TurboTax will ask for or need your year-end balance.
