HI,
Each new conversion starts its own five-year clock, and you'll need to account for multiple conversions to make sure you don't take out too much money too soon. Note that the five-year rule applies equally to Roth conversions for both pre-tax and after-tax funds in a traditional IRA.
This article may also help with additional questions.
https://blog.turbotax.intuit.com/retirement/roth-ira-withdrawal-rules-and-penalties-53233/
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Best,
Karen
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