Yes, because it sounds like your direct Roth 401K was a designated Roth 401K. According to the Turbo Tax on demand tax guidance, this could also represent the basis you have in your designated Roth account. The designated Roth after tax contributions may be taken out tax-free.
It could also be the portion of your insurance premiums you paid that can be taken out tax-free. Another entry could be the nontaxable portion of a charitable gift annuity.
You might wish to contact the trustee of your Roth account to find out what the is basis represents and why it was reported in Box 5 of the 1099R.
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