ISO exercised: loss harvesting or carrying over

In 2021, my exercised ISOs those were granted in 2017. The FMV was $0 and exercise price was $0.5. Say 100 stocks were exercised. While filing 2021 taxes, AMT was 0.

 

In 2023, the startup ceased/closed its business. Other than a a letter saying business ceased, we did not receive any tax related documents.

 

Can we harvest this $50 loss? How do I calculate the loss (I assume AMT credit)? How do I file this in turbotax?