Yes, because you took the standard deduction in 2020, any itemized deductions you would have used will not carry over to another year.
In 2020, the standard was $12,400 for a single person and $24, 800 for married filing jointly. When you entered your itemized information, it sounds like TurboTax saw that your itemized numbers were less than the standard.
The $300 deduction for cash donations was part of the tax changes for that specific tax year and in addition to the standard deduction.
When you do itemize, sometimes you can't take the full deduction for your donations because of income limitations. When this happens, you can carry the unused amount forward to another year. However, this is only if you itemize that year.
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