Yes, as long as your Schedule C business shows a net profit and the health insurance premium does not exceed that net profit, the amount paid (by either of you) for your spouse's health insurance premiums can be used as a self-employed health insurance deduction.
The insurance must not have been purchased through a Health Insurance Marketplace and neither you nor your spouse were qualified to be covered under an employer health plan.
The self-employed health insurance deduction is entered through the Schedule C section of your return.
If you are using the TurboTax Online Self-Employed version, look in the Less Common Expenses section of the Schedule C. Once you have expanded that section, look for the Health Insurance Premiums category to enter your information.
If you are using the CD/downloaded Home and Business version of TurboTax, you will find the entry in the Schedule C section, then Business Expenses > Other Common Business Expenses > Insurance Payments > Health Insurance Premiums.
Take a look at the following TurboTax article for more details: Can I deduct health insurance premiums if I'm self-employed?
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