You've indicated that you are using TurboTax Home and Business. If this is the case, your business is either a sole-proprietor, partnership, or single-member LLC. As such, the information I've provided pertains to that business structure.
In general, charitable contributions are not allowed as a business expense on the Schedule C. However, if you made a payment to a charitable organization that was not for charity nor a gift, you may deduct the payment as an expense to the business. You may want to clarify or consider the terms of your donation. If your business received any benefit for the donation (ad space or marketing, for example) you may deduct the payment as an expense. Otherwise, your charitable gift/donation is not deductible under your business, but may be deducted on the Schedule A if you itemize your deductions for your personal tax return.
You may want to review the IRS link below for clarification on this rule. There are examples that may help you determine if you can deduct the payments or not: http://www.irs.gov/publications/p535/ch11.html.
