After you file

owed over $35,000 in taxes in 2020.    Do you mean in 2020, but for 2019?  Or for 2020?  If the former, see this:

IRS Backlog: 2.4 Million 2019 Tax Returns Still In Pipeline (forbes.com)

 

$35,000?  Or do you mean $3,500?  Or $350?  If you really owe $35,000 on a personal tax return, the IRS is likely to pull your filing for a manual review.  In general, though, unless you indicated a specific date, the payment is pulled on the due date of the return.  In either case, it can still take the government a few days to do this.