To follow-up on the comments from @RobertB4444, fluctuations in the share price do not affect cost basis. Regarding adjusting your basis on the first sale of GLD, you can adjust your basis on the page in TurboTax where you elect to manually enter your trade information. In TurboTax online, go to the Income & Expenses section, scroll down to the Stocks, Cryptocurrency, Mutual Funds, etc, subsection, select Start or Review, and then find the GLD transaction that you need to edit.
Regarding the sale of your GLD, there may have been GLD expenses you incurred while you held those shares which you eventually sold. Those GLD expenses are miscellaneous itemized deductions, which are not deductible for U.S. federal income tax purposes by individuals in 2021.
Regarding the GLD that you currently own, the Trust issued a 2021 Reporting Statement to shareholders of record. Pages 11 and 12 of that Reporting Statement provide a Step-by-Step analysis of a hypothetical shareholder who purchased GLD during 2021 and held the shares for the remainder of the year. The analysis explains how to calculate the shareholder's adjusted gold held and cost basis.
Here is a link to the GLD 2021 Reporting Statement.
2021 GLD Reporting Statement
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