It depends. If I understand you correctly, you already claimed the capital gains exclusion in the year you sold the home. If this is true, then you cannot reclaim it in a following year when your filing status changed..
Now if you amend your original return in the year you sold the home changing your filing status from Married Filing Jointly to Married Filing Separately, then you are permitted to do that if you meet the ownership and use test outlined in the following link. If this is the case, you can each exclude up to $250,000 each.
There are exceptions to the eligibility tests where you can qualify for the exclusion. Please review IRS publication 523 at the following link for a list of these exceptions.
If you need to amend, please refer to the following Turbo Tax article that summarizes the amendment process.
**Say "Thanks" by clicking the thumb icon in a post
**Mark the post that answers your question by clicking on "Mark as Best Answer"
