Yes, if your wife had self-employment income and expenses to claim for 2020 then you will need to use the TurboTax Online Self-Employed version or one of the CD/downloaded versions of TurboTax.
The self-employed version supports all of the forms necessary to report Schedule C income and deductions as well as the applicable Self-Employment tax if the business was profitable.
Additionally, if your wife is no longer self-employed or actively pursuing her Schedule C business, you will need to indicate that the business ended in 2020. If there were any assets being depreciated through the business, you will also need to indicate that those are no longer used for business purposes.
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