Yes, you will need an ITIN for anyone listed on your tax return. For details on how to do so, click these links for Form W-7 and Instructions for Form W-7.
I'm also including these links to IRS Publication, 519 U.S. Tax Guide for Aliens and IRS Publication 54, Tax Guide for U.S. Citizens and Resident Aliens Abroad.
Note that once you have met the test to be treated as a resident alien for tax purposes (Green Card or Substantial Presence Test), you have the option to have your nonresident spouse treated as a resident and file a joint tax return. This includes situations in which one spouse is a nonresident alien at the beginning of the tax year, but a resident alien at the end of the year, and the other spouse is a nonresident alien at the end of the year.
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