Yes, you would need to enter all of the donations made in 2025 on the 2025 return to make use of the 5-year carryover period. If you determine that you have more than you would use in 5 years, then you could opt not to enter the excess donations.
If you anticipate making another large donation in the future, it may be easier to make donations annually.
See this tax tips article for more information about charitable contribution deductions.
See this help article if you need to enter carryover donations from a previous year when you didn't use TurboTax.
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