Yes, if you make a non-deductible contribution to an IRA, then a backdoor Roth conversion, this will trigger Form 8606 in your return. You'll be asked about any IRA basis you have. If you had no other non-deductible IRA contributions, your beginning basis would be $0.
Here's detailed instructions on How to Enter a Back-Door Roth Conversion, which is a two-step process.
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