You can only deduct expenses incurred for earning the income reported on the Form 1099-NEC as self-employment income. You will report this income and any related expenses on Schedule C. After entering the Form 1099-NEC, TurboTax will walk you through entering any expenses you incurred to earn that money.
Any expenses related to earning the income reported on the W-2 are not deductible for federal purposes. However, there are a few states that allow a deduction for these expenses on the state tax return. See this TurboTax Help article for more details: Can employees deduct any job-related expenses?
For more information regarding self-employment income, check out these TurboTax Help Articles:
What is a Form 1099-NEC?
Where do I enter Schedule C?
What self-employed expenses can I deduct?
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