Yes.
According to the IRS:
“Also, you may be able to increase your exclusion amount from $250,000 to $500,000. You may take the higher exclusion if you meet all of the following conditions.
- You sell your home within 2 years of the death of your spouse;
- You haven’t remarried at the time of the sale;
- Neither you nor your late spouse took the exclusion on another home sold less than 2 years before the date of the current home sale; and
- You meet the 2-year ownership and residence requirements (including your late spouse's times of ownership and residence, if applicable).”
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