Yes, you can use the preschool tuition to claim the Child and Dependent Care Credit. See more below.

https://ttlc.intuit.com/replies/4396093

To Enter the Credit in TurboTax Online:

1. Go to "Federal Taxes" at the top of the screen

2. Select "Deductions & Credits"

3. Scroll down to "You and Your Family" and select "Show More"

4. Select "Start" next to "Child and Dependent Care Credit"

5. Select "Yes" to "Did you pay for child or dependent care in 2016?"

6. Select "Continue" on the Add Dependents page and proceed to enter your info for the credit