If you are self-employed and took the course to increase your knowledge in your profession, then you can deduct the cost of the course.

You can treat this as a "continuing education" expense in Other miscellaneous expenses.

If this training was for an outside job, it may be deductible as an unreimbursed job-related expense.  Unreimbursed job-related expenses are deductible on Schedule A (Itemized Deductions) and are subject to the 2% floor for miscellaneous itemized deductions.  To input your job-related expenses (TurboTax Deluxe edition or higher is required to file itemized deductions):