If the child care was deducted from your gross pay such that it is not included in your Box 1 of Form W-2 in the first place, then you can get no further tax benefit from it.

If it is not a pre-tax deduction as above, then, No, child care is not deductible.  However, you may qualify for the Child and Dependent Care Credit if you need the child care so you (and your spouse, if married) can work or go to school.  The CDCC is a percentage of your qualifying child care expenses based on your adjusted gross income.  TT has a separate interview section for the CDCC that you should complete after you have entered all your income items and personal information, including dependent info.  The CDCC reduces your tax liability but cannot reduce it below zero.